To assess weight loss, mice were first fattened on HFD and then assigned to normal chow plus or minus NR. Supplemented and nonsupplemented mice were also randomized to 5 days/week forced exercise or not. NR and exercise promoted weight loss individually and in an additive fashion. To assess the mechanism by which NR promotes weight loss, we measured calorie intake, activity, calorie waisted, resting metabolic rate, body composition, glucose tolerance, insulin sensitivity, and a variety of biochemical and metabolomic parameters related to liver mitochondrial function.

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